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KMID : 0811720000040000012
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Korean Journal of Physiology & Pharmacology
2000 Volume.4 No. 0 p.12 ~ p.0
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Using cDNA Microarray Technology to Study the Cortical Neuronal Stem Cell Differentiation
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Kim Jin-Hyuk
Kim Hye-Young
Shin Hong-Kee
Kim Ki-Soon
Lee Yong-Sung
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Abstract
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The emergence of cDNA microarray-based technologies has revolutionized the analysis of gene expression and DNA sequence. They have made it possible to survey several thousands of genes in a single experiment. In the case of neuronal differentiation, there have been many reports on the genes to be changed in their expression rates during neuronal differentiation. However, entire process or relationship between each process during differentiation of neuron is not clear yet.
The cortical neuronal stem cells were isolated from rat fetal brain (embryonic day 15.5) and differentiation of them was prevented with bFGF (basic fibroblast growth factor) included in serum free media. Neuronal differentiation was induced by removal of bFGF from culture media and RNA was isolated to hybridize to a cDNA microarray which has over 2,400 known and 1,700 unknown genes isolated from developing fetal rat brain. Scanned images of cDNA microarray chips were analyzed with Imagene (Biodiscovery Co.) and MAAS (Microarray Analysis System; home made). Changes of gene expression pattern along neuronal stem cell differentiation was investigated with k-means clustering.
Expression levels of many genes such as sodium. potassium and calcium ion channels and several transporters were increased after several hours from the induction of differentiation. Results must be confirmed with other experimental techniques and path finding should be achieved in the future.
Source: Korean Journal of Physiology & Pharmacology.2000 Oct;4(Suppl):S18-S18
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